ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (12): 2258-2263.doi: 10.11843/j.issn.0366-6964.2015.12.018

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Prokaryotic Soluble Expression of Truncated SAG2 Protein of Toxoplasma gondii and Its Antigenic Activity

ZHAO Dong-yue*,CHEN Jin-feng,WEI Jian-hui   

  1. (Southern Biomedical Research Center at Fujian Normal University,Fuzhou 350117,China)
  • Received:2014-10-11 Online:2015-12-23 Published:2015-12-23

Abstract:

To express SAG2 gene of Toxoplasma gondii by prokaryotic expression system and to identify its antigenicity,truncated SAG2 without the highly hydrophobic signal peptide and C-terminus were amplified by PCR,and recombinant prokaryotic expression plasmid (pGEX-4T-3-SAG2t) with SAG2t protein gene was constructed.Then the recombinant plasmids were transferred into E.coli DH5α,and the bacteria were induced by IPTG.The expression and immune-reactivity of SAG2t protein were detected by SDS-PAGE and Western blotting respectively.The yield of purified rSAG2t was more than 4 mg per liter of culture medium.Western blot showed that rSAG2t can be strongly recognized by mice serum infected with T.gondii ME49 strain and mice serum immunized by rSAG2t .The result showed that the soluble expression of SAG2t protein with favorable complete antigenic activity was implemented by prokaryotic expression system.

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