ZHAO Dong-yue,CHEN Jin-feng,WEI Jian-hui. Prokaryotic Soluble Expression of Truncated SAG2 Protein of Toxoplasma gondii and Its Antigenic Activity[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2258-2263.
[1]GUTIERREZ J,O’DONOVAN J,WILLIAMS E,et al.Detection and quantification of Toxoplasma gondii in ovine maternal and foetal tissues from experimentally infected pregnant ewes using real-time PCR[J].Vet Parasitol,2010,172(1-2):8-15.
[2]PUTIGNANI L,MANCINELLI L,DEL CHIERICO F,et al.Investigation of Toxoplasma gondii presence in farmed shellfish by nested-PCR and real-time PCR fluorescent amplicon generation assay (FLAG)[J].Exp Parasitol,2011,127(2):409-417.
[3]LYONS R E,LYONS K,MCLEOD R,et al.Construction and validation of a polycompetitor construct (SWITCH) for use in competitive RT-PCR to assess tachyzoite-bradyzoite interconversion in Toxoplasma gondii[J].Parasitology,2001,123(5):433-439.
[4]YANG C D,CHANG G N,CHAO D.Protective immunity against Toxoplasma gondii in mice induced by a chimeric protein rSAG1/2[J]. Parasitol Res,2004,92(1):58-64.
[5]MACHADO A V,CAETANO B C,BARBOSA R P,et al.Prime and boost immunization with influenza and adenovirus encoding the Toxoplasma gondii surface antigen 2 (SAG2) induces strong protective immunity[J].Vaccine,2010,28(18):3247-3256.
[6]TOMAVO S,MARTINAGE A,DUBREMETZ J F.Phosphorylation of Toxoplasma gondii major surface antigens[J]. Parasitol Res,1992,78(7):541-544.
[7]龙彩虹,吴少庭,翁亚彪,等.弓形虫表面抗原 SAG2 基因片段的克隆与原核表达[J].中国人兽共患病杂志,2003,19(2):42-45.
LONG C H,WU S T,WENG Y B,et al.Cloning and prokaryotic expression of SAG2 gene of Toxoplasma gondii [J]. Chinese Journal of Zoonoses,2003,19(2):42-45.(in Chinese)
[8]雷明军,吴少庭,戴五星,等.弓形虫 RH 株膜表面抗原 2 全长基因的高效表达与抗原性分析[J].中国寄生虫学与寄生虫病杂志,2004,22(4):231-234.
LEI M J,WU S T,DAI W X,et al.High efficiency expression and antigenicity analysis of the SAG2 gene from Toxoplasma gondii RH strain[J]. Chinese Journal of Parasitology and Parasitic Diseases,2004,22(4):231-234.(in Chinese)
[9]黄燕,徐梅倩,崔立,等.刚地弓形虫抗原基因 SAG2 的克隆、表达和纯化[J].中国兽医寄生虫病,2007,15(6):8-12.
HUANG Y,XU M Q,CUI L,et al.Cloning,expression and purification of SAG2 gene of Toxoplasma gondii [J].Chinese Journal of Veterinary Parasitology,2007,15(6):8-12.(in Chinese)
[10]聂福旭,蒋蔚,王权,等.弓形虫膜表面抗原 SAG2 蛋白的高效表达及免疫活性分析[J].生物技术通报,2010(4):173-178.
NIE F X,JIANG W,WANG Q,et al.Expression and immunological activity analysis on the surface protein SAG2 from Toxoplasma gondii strain in RH[J].Biotechnology Bulletin,2010(4):173-178.(in Chinese)
[11]LI S,GALVAN G,ARAUJO F G,et al.Serodiagnosis of recently acquired Toxoplasma gondii infection using an enzyme-linked immunosorbent assay with a combination of recombinant antigens[J].Clin Diagn Lab Immunol,2000,7(5):781-787.
[12]LAU Y L,SHAMILAH H,FONG M Y.Characterisation of a truncated Toxoplasma gondii surface antigen 2 (SAG2) secreted by the methylotrophic yeast Pichia pastoris[J].Trop Biomed,2006,23(2):186-193.
[13]LAU Y L,FONG M Y.Toxoplasma gondii:serological characterization and immunogenicity of recombinant surface antigen 2 (SAG2) expressed in the yeast Pichia pastoris[J].Exp Parasitol,2008,119(3):373-378.